Journal: The Journal of veterinary medical science
Article Title: Effect of skin fibroblast-derived allogeneic feeder cells on porcine ES-like cell establishment.
doi: 10.1292/jvms.11-0301
Figure Lengend Snippet: Fig. 1. Morphology of mitotically inactivated feeder cells derived from porcine ear skin fibro- blasts (PESF, A), porcine tail skin fibroblasts (PTSF, B), human foreskin fibroblasts (HFK2429, C), CF1-mouse embryonic fibroblasts (CF1-MEF, D) and a continuous SIM mouse embryo- derived thioguanine- and ouabain-resistant cell line (STO, E). Scale bar =100 µm.
Article Snippet: Isolation and culture of feeder cells: Five types of fibro- blast cells, including porcine ear skin fibroblasts (PESF), porcine tail skin fibroblasts (PTSF), a human foreskin fibroblast cell line (HFK; CRL-2429, ATCC), a continuous SIM mouse embryo-derived thioguanine- and ouabain- resistant cell line (STO; CRL-1503, ATCC) and a CF1 mouse embryonic fibroblast cell line (CF1-MEF; CRL-1040, ATCC), were used as feeder cells in order to compare their efficiency in terms of derivation of pES-like cells (Fig. 1).
Techniques: Derivative Assay